Mangifera indica Linn (Anacardiaceae) commonly called as “A¬m” in Hindi is used in treatment of syphilis, emesis, inflammation, ulcer, rheumatism and diabetes ethnopharmacologically. Mangifera indica bark was extracted with solvents of increasing polarity viz. petroleum ether, chloroform and methanol successively by continuous hot percolation method. The anti-arthritic potential of Mangifera indica methanol extract was then evaluated using three different in-vitro models viz. inhibition of denaturation of protein, proteinase and hyaluronidase. The concentrations of 10 to 200 μg/ml of Mangifera indica methanol extract were prepared using DMSO. Diclofenac was used as the positive control. All determinations were done in triplicate. A dose dependent increase in percentage inhibition was observed for all the three models. The inhibitory concentration (IC50) was found to be 70 μg/ml for protein denaturation assay, 32 μg/ml in proteinase inhibitory action and 18 μg/ml for anti-hyaluronidase activity. Diclofenac offered protective activity at even much lower concentrations compared to Mangifera indica methanol extract produced IC50 values of 40 and 13 μg/ml in protein denaturation and proteinase inhibitory assays. Mangifera indica exhibited a very good anti-arthritic activity in all the tested models confirming its folklore use. In-vivo studies are needed to confirm its anti-arthritic activity and further explore its possible mechanism and identify the desired phytoconstituents which protects from this autoimmune disease.
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